If you’ve ever tried measuring a precise volume of a liquid in your laboratory, you’ve probably used a graduated pipette at least once. Whether you’re mixing reagents for an experiment or preparing a standard solution for a chemistry practical, a graduated pipette is often the tool that ensures the exactness you demand.
Graduated pipettes are available in various classes, standards, and configurations to suit different precision levels and regulatory requirements. They may seem simple at first glance, but there’s a lot to consider if you want to get the most out of them, from choosing the right class of accuracy to understanding the marking techniques and calibration standards.
A graduated pipette is a long, narrow glass marked with a series of graduations for measuring and transferring liquids. Unlike volumetric pipettes, which are calibrated to measure a single fixed volume, graduated pipettes offer flexibility with their multiple-volume markings.
These pipettes are commonly used in:
When exploring pipettes, you’ll encounter different classes, most notably Class A and Class B. Class A pipettes offer the highest accuracy and are calibrated to stringent standards. They come with calibration certificates that demonstrate traceability to national standards, making them ideal for critical analyses. Class B pipettes, while still accurate, have slightly broader tolerance limits and are often suited for routine laboratory work where top-tier precision is not as critical.
Graduated pipettes come in several varieties, each designed for specific tasks and compliance standards. Some pipettes measure liquids from a zero mark downward (Mohr pipettes), while others are designed to deliver the total volume down to the tip (Serological pipettes).
| Feature | Class A Serological Pipettes | Class B Serological Pipettes | Class A/ Class BMohr Pipettes | High-Capacity Stirrer |
| Calibration | To the tip | To the tip | Between graduations | Industrial-scale operations |
| Marking Colour | Amber | Amber/ White | Amber/ White | Up to 20 litres |
| Typical Use | High-precision tasks | Routine applications | Partial volume transfers | Hermetic construction |
| Temperature Calibration | 20°C/ 27°C | 20°C/ 27°C | 20°C/ 27°C | Precise temp regulation |
Graduated pipettes find applications in diverse scientific fields due to their versatility and precision.
In chemical analysis, graduated pipettes are indispensable for preparing standard solutions, performing titrations, and accurately diluting samples. Their flexibility allows researchers to handle varying liquid volumes effortlessly.
Pharmaceutical laboratories rely on graduated pipettes to maintain the accuracy of drug formulation and quality control. For instance, Class A pipettes, which come with individual calibration certificates, ensure compliance with stringent regulatory standards.
In cell culture and microbiology, graduated pipettes are used for transferring precise amounts of reagents, media, or samples. Their ability to handle small volumes makes them ideal for delicate processes.
Graduated pipettes play a key role in water quality testing and soil analysis by enabling precise liquid handling for accurate measurements.
Graduated pipettes are designed to offer precision and durability. Some key features include:
For example, the 7079 Class A Serological Pipette from Borosil Scientific combines all these features, making it a reliable choice for high-accuracy tasks.
Proper care extends the lifespan of graduated pipettes and ensures consistent performance. Follow these tips for optimal use.
The flexibility, precision, and ease of use offered by graduated pipettes make them an essential tool in any laboratory. Their applications span multiple disciplines, making them a universal solution for liquid handling tasks. When paired with high-quality materials and robust calibration, these pipettes ensure accurate and reproducible results, allowing researchers to focus on their experiments without concern for measurement errors.
Graduated pipettes might seem like simple glass tools, but their accuracy, compliance standards, and calibration details can make a big difference in your laboratory work. Understanding the differences between Class A, Class B, Serological, and Mohr pipettes can help you select the perfect instrument for your specific needs. Investing in well-calibrated, durable pipettes ensures that your lab work remains consistent, reproducible, and trustworthy.
At Borosil Scientific, we understand the importance of accuracy and durability in laboratory tools. Our range of serological and Mohr pipettes, backed by compliance with ASTM, USP, IS, and ISO standards, ensure you have the right tool for every job. Contact us to learn how we can support your laboratory’s success with precision-crafted pipettes.
