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How to Use the Different Types of Pipettes

How to Use the Different Types of Pipettes

What are pipettes?

Pipettes are more than just tools. They play a crucial role in ensuring accurate liquid handling in any well-equipped laboratory. From quality control labs in pharmaceutical plants to life sciences and academic research, pipettes play a significant role in day-to-day workflows. However, choosing the right one and, more importantly, using it correctly, ensures your positive results in any experiment.

This article breaks down the different types of pipettes, explains how each one works, and identifies what makes them suitable for specific lab tasks. All information here is drawn from certified, laboratory-validated glassware.

Volumetric Pipettes: High Precision for a Single Volume

When the task involves transferring a fixed, precise volume, volumetric pipettes are the gold standard. They’re calibrated to deliver (TD) a specific volume, making them ideal for quantitative analysis.

Volumetric Pipettes

  • Accuracy Class: Class A
  • Certification: NABL certified with individual calibration certificate
  • Material: 3.3 Borosilicate Glass
  • Calibrated at 27°C (tropical standard)

How to Use

  • Rinse the pipette with the liquid before use to condition the surface.
  • Fill using a pipette bulb or dispenser until the bottom of the meniscus aligns with the calibration mark.
  • Do not blow out the last drop; the design compensates for that.
  • Hold it vertically during measurement to avoid parallax errors.

Volumetric pipettes are widely used for preparing standard solutions in analytical chemistry. Borosil Scientific’s Class A certified volumetric pipettes offer traceability, accuracy, and chemical durability, essential for labs under compliance pressure.

Mohr Pipettes: Graduated for Volume Flexibility

Mohr pipettes allow the transfer of different volumes, with graduations starting above the tip. These are not as precise as volumetric pipettes, but they are ideal when flexibility is more important than absolute accuracy.

Mohr Pipettes

  • Class: A
  • Certification: NABL Certified
  • Design: Clear graduation, partial delivery type
  • Material: 3.3 Borosilicate glass

How to Use?

  • Always read and measure between two graduation marks.
  • Avoid draining to the tip; these pipettes are not designed for full-volume delivery.
  • Rinse before use and drain slowly to prevent variations in volume.

In quality control routines where volumes vary slightly between processes, such as in food and beverage laboratories or during reagent transfers, Mohr pipettes provide a reliable and accurate solution without the need for multiple volumetric pipettes.Micropipettes: Sub-Millilitre Precision

For anything under 1 mL, especially in microlitre ranges, micropipettes are the go-to solution. These are most commonly used in clinical diagnostics, molecular biology, and pharmaceutical research and development.

LabQuest’s C3 Micropipette Series:

Volume RangeFeaturesCertification
1– 10 µLHigh precision, easy maintenanceISO 8655
10 – 200 µLErgonomic grip, intuitive controlISO 8655
20 – 10000 µLBalanced comfort, consistent outputISO 8655

Handling Best Practices

  • Use only compatible tips and change them between samples.
  • Always aspirate and dispense vertically.
  • Pre-wet tips for viscous liquids or temperature-sensitive samples.
  • Press the plunger to the first stop to aspirate, then to the second stop to dispense.

Micropipette errors often stem from poor technique, rather than the instrument itself. Borosil Scientific’s micropipettes feature smooth plunging mechanisms and well-calibrated controls, supporting repetitive pipetting with minimal hand fatigue.

Gerber Milk and Bacteriological Pipettes

Specialised pipettes, like those used in milk testing, require strict standardisation. These are calibrated according to established dairy testing methods and are part of Borosil Scientific’s dairy lab glassware range.

Recommended Use

  • Used in the Gerber method for fat content determination in milk.
  • Preheat the pipette before adding sulfuric acid and milk to maintain consistent results.
  • Always dry thoroughly after cleaning; do not store wet.

Labs using these should always verify calibration integrity, especially when results are included in regulatory reporting.

Drum Sampling and Artificial Insemination Pipettes

Drum sampling pipettes are used to withdraw liquid samples from chemical drums or large containers. These are designed for robustness and chemical resistance, especially when samples are collected from heights or depths.

Artificial insemination pipettes, on the other hand, are specifically designed for veterinary labs and require sterility and a consistent bore size. These are usually single-use or autoclavable.

Borosil Scientific offers these pipettes based on requirement-specific sourcing. They are not part of standard glassware distribution but can be provided under industrial order flows.

Calibration and Maintenance: Non-Negotiable for Accuracy

Every pipette, whether it’s Class A glassware or a variable micropipette, requires scheduled calibration to remain accurate.

Recommended Calibration Schedule:

  • Daily use: Calibrate every 3 months
  • Moderate use: Calibrate every 6 months
  • Infrequent use: Once a year is acceptable

Common Pitfalls and Fixes:

  • If you’re getting inconsistent results, pre-wet the pipette and rinse before use.
  • If you see air bubbles in micropipettes, slow down the plunger’s release.
  • If there’s a volume drift over time, check calibration with gravimetric testing.
  • If you find frequent residue buildup, use dedicated cleaning solutions and dry at low heat (60–80°C).

Borosil Scientific includes calibration documentation wherever applicable and provides individual or batch certification in accordance with ISO and NABL standards.

Technical Parameters to Consider Before Pipette Selection

1. Volume Range:

Always select the pipette with the lowest upper limit that can still accommodate your required volume. For example, use a 2–20 µL micropipette instead of a 100–1000 µL one for 10 µL samples.

2. Material Compatibility:

Borosil Scientific pipettes are made from 3.3 borosilicate glass; Hence, you should avoid use with HF or hydrofluoric acid. For those, polymer-based products are used under controlled conditions but are not part of Borosil Scientific’s current manufacturing.

3. Certification Needs:

If your process falls under audit, use pipettes with traceable certification (e.g., 2040 Volumetric Pipette with individual COA or 7058 Milk Pipette with dairy lab compliance).

4. Operator Comfort:

Repetitive pipetting tasks (as seen in diagnostic labs) demand ergonomic models like the C2 and C3 micropipettes, which reduce fatigue.

Conclusion 

Whether you are dispensing 10 µL of buffer into a PCR tube or measuring 11 mL of milk for fat testing, choosing the correct pipette is crucial. The type of pipette you use and how you use it can influence everything from product quality to audit success.

At Borosil Scientific, we offer certified, accurate, and ergonomically designed pipettes across various categories, ranging from Class A volumetric glass pipettes to comfort-fit micropipettes, each designed to deliver confidence in every drop.

If your lab requires precision, traceability, and long-term value, please speak with our team or browse our comprehensive catalogue to select the ideal pipette for your application.

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